Analysis Note

ControlTonsil, human skin tissue

Application

Research Sub CategoryIntegrins

Anti-Integrin β1 Antibody, clone 6S6, Azide Free detects level of Integrin β1 & has been published & validated for use in FC, IH & IP.

Research CategoryCell Structure

Immunoprecipitation

Immunohistochemistry on frozen sections

Flow cytometry

Blocks adhesion of cells to extracellular matrix proteins

Induces aggregation of certain lymphoid cells

Does not bind reduced beta.1 integrin on Western blot

Optimal working dilutions must be determined by end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Immunogen

Human synovial cells

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Protein A Purified mouse immunoglobulin in PBS, no preservatives.

Format: Purified

Specificity

Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Similar in behavior and characteristics to antibody clone 3S3 (Wilkins et al., 1996).

Storage and Stability

Maintain 6 months at 4°C, from date of shipment. Do not freeze. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Target description

88 kDa